PPP3CB polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PPP3CB.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PPP3CB.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of PPP3CB polyclonal antibody (Cat # PAB2160) in mouse kidney tissue lysate. PPP3CB (arrow) was detected using the purified Polyclonal antibody.Western Blot (Cell lysate)
Western blot analysis of PPP3CB polyclonal antibody (Cat # PAB2160) in Jurkat cell line lysates (35 ug/lane). PPP3CB (arrow) was detected using the purified Polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PPP3CB polyclonal antibody (Cat # PAB2160) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — PPP3CB
Entrez GeneID
5532Protein Accession#
NP_066955;P16298Gene Name
PPP3CB
Gene Alias
CALNA2, CALNB
Gene Description
protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform
Omim ID
114106Gene Ontology
HyperlinkGene Summary
catalytic subunit
Other Designations
OTTHUMP00000019823|calcineurin A beta|calcineurin A2|protein phosphatase from PCR fragment H32
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Interactome
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Publication Reference
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Calcineurin inhibits Na+/Ca2+ exchange in phenylephrine-treated hypertrophic cardiomyocytes.
Katanosaka Y, Iwata Y, Kobayashi Y, Shibasaki F, Wakabayashi S, Shigekawa M.
The Journal of Biological Chemistry 2005 Feb; 280(7):5764.
Application:IF, IHC-Fr, IP, WB, Mouse , Brains, Cardiomyocytes, Hearts.
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The protein phosphatase calcineurin determines basal parathyroid hormone gene expression.
Bell O, Gaberman E, Kilav R, Levi R, Cox KB, Molkentin JD, Silver J, Naveh-Many T.
Molecular Endocrinology (Baltimore, Md.) 2005 Feb; 19(2):516.
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Calcineurin inhibits Na+/Ca2+ exchange in phenylephrine-treated hypertrophic cardiomyocytes.
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