MLF1IP polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant MLF1IP.
Immunogen
Recombinant protein corresponding to amino acids of human MLF1IP.
Sequence
EETYETFDPPLHSTAIYADEEEFSKHCGLSLSSTPPGKEAKRSSDTSGNEASEIESVKISAKKPGRKLRPISDDSESIEESDTRRKVKSAEKISTQRHEVIRTTASSELSEKPAESV
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebellum with MLF1IP polyclonal antibody (Cat # PAB21473) shows strong cytoplasmic and nuclear positivity in Purkinje cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with MLF1IP polyclonal antibody (Cat # PAB21473) at 1-4 ug/mL dilution shows positivity in nucleus, cytoplasm and centrosome. -
Gene Info — MLF1IP
Entrez GeneID
79682Protein Accession#
Q71F23Gene Name
MLF1IP
Gene Alias
CENP-50, CENP-U, CENP-U(50), CENP50, CENPU, FLJ23468, KLIP1, PBIP1
Gene Description
MLF1 interacting protein
Omim ID
611511Gene Ontology
HyperlinkGene Summary
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM
Other Designations
KSHV latent nuclear antigen interacting protein 1|centromere protein U|polo-box-interacting protein 1
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Interactome
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