PIP5K1C polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The PIP5K1C polyclonal antibody (Cat # PAB2133) is used in Western blot to detect PIP5K1C in HeLa cell lysate.
Western Blot (Transfected lysate)
Western blot analysis of PIP5K1C (arrow) using PIP5K1C polyclonal antibody (Cat # PAB2133). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K1C gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PIP5K1C polyclonal antibody (Cat # PAB2133) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PIP5K1C.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PIP5K1C.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The PIP5K1C polyclonal antibody (Cat # PAB2133) is used in Western blot to detect PIP5K1C in HeLa cell lysate.Western Blot (Transfected lysate)
Western blot analysis of PIP5K1C (arrow) using PIP5K1C polyclonal antibody (Cat # PAB2133). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K1C gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PIP5K1C polyclonal antibody (Cat # PAB2133) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — PIP5K1C
Entrez GeneID
23396Protein Accession#
NP_036530;O60331Gene Name
PIP5K1C
Gene Alias
KIAA0589, LCCS3, PIP5K-GAMMA, PIP5Kgamma
Gene Description
phosphatidylinositol-4-phosphate 5-kinase, type I, gamma
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the type I phosphatidylinositol-4-phosphate 5-kinase family of enzymes. A similar protein in mice is found in synapses and focal adhesion plaques, and binds the FERM domain of talin through its C-terminus. [provided by RefSeq
Other Designations
Human homolog of mouse phosphatidylinositol-4-phosphate 5-kinase I-gamma|PtdIns(4)P-5-kinase|diphosphoinositide kinase|phosphatidylinositol-4-phosphate 5-kinase I-gamma|type I PIP kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Type I gamma phosphatidylinositol phosphate kinase targets and regulates focal adhesions.
Ling K, Doughman RL, Firestone AJ, Bunce MW, Anderson RA.
Nature 2002 Nov; 420(6911):89.
Application：IF, IP, IP-WB, WB-Ce, Human, Mouse, SH-Sy5y, PC-12, MCF-7, U2OS, NRK, 3T3, 293T cells, Brain.
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Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the third isoform and deletion/substitution analysis of members of this novel lipid kinase family.
Ishihara H, Shibasaki Y, Kizuki N, Wada T, Yazaki Y, Asano T, Oka Y.
The Journal of Biological Chemistry 1998 Apr; 273(15):8741.
Application：WB-Ti, WB-Tr, Mouse, Monkey, COS-7 cells, Brain.
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Type I gamma phosphatidylinositol phosphate kinase targets and regulates focal adhesions.
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