AASS polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant AASS.
Immunogen
Recombinant protein corresponding to amino acids of human AASS.
Sequence
MLQVHRTGLGRLGVSLSKGLHHKAVLAVRREDVNAWERRAPLAPKHIKGITNLGYKVLIQPSNRRAIHDKDYVKAGGILQEDISEACLILGVKRPPEEKLMSRKTYAFFSHTIKAQEANMGLLDEILKQEIR
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with AASS polyclonal antibody (Cat # PAB21329) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human liver with AASS polyclonal antibody (Cat # PAB21329) shows strong cytoplasmic positivity in hepatocytes at 1:200-1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with AASS polyclonal antibody (Cat # PAB21329) at 1-4 ug/mL dilution shows positivity in cytoplasm and mitochondria. -
Gene Info — AASS
Entrez GeneID
10157Protein Accession#
Q9UDR5Gene Name
AASS
Gene Alias
LKR/SDH, LKRSDH, LORSDH
Gene Description
aminoadipate-semialdehyde synthase
Gene Ontology
HyperlinkGene Summary
This gene encodes a bifunctional enzyme that catalyzes the first two steps in the mammalian lysine degradation pathway. The N-terminal and the C-terminal portions of this enzyme contain lysine-ketoglutarate reductase and saccharopine dehydrogenase activity, respectively, resulting in the conversion of lysine to alpha-aminoadipic semialdehyde. Mutations in this gene are associated with familial hyperlysinemia. [provided by RefSeq
Other Designations
alpha-aminoadipate semialdehyde synthase|aminoadipic semialdehyde synthase|lysine-2-oxoglutarate reductase|lysine-ketoglutarate reductase /saccharopine dehydrogenase
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Interactome
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Pathway
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