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Last updated: 2016/12/4
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PI4K2A polyclonal antibody

  • Catalog # : PAB2131
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PI4K2A.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PI4K2A.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:50-100)
    Immunofluorescence (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of PI4K2A polyclonal antibody (Cat # PAB2131) in HL-60 cell lysate. PI4K2A (arrow) was detected using purified Polyclonal antibody.
    Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PI4K2A polyclonal antibody (Cat # PAB2131) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
    This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
  • Immunofluorescence
  • Immunofluorescence
  • A : Negative control of HeLa cells without Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence.
    B : Immunofluorescence analysis of PI4K2A polyclonal antibody (Cat # PAB2131) in HeLa cells.
    Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Protein Accession#:
  • NP_060895;Q9BTU6
  • Gene Name:
  • PI4K2A
  • Gene Alias:
  • DKFZp761G1923,PI4KII,PIK42A,RP11-548K23.6
  • Gene Description:
  • phosphatidylinositol 4-kinase type 2 alpha
  • Gene Summary:
  • Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000020224,phosphatidylinositol 4-kinase type II (PI4KII)
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