PI4K2A polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of PI4K2A polyclonal antibody (Cat # PAB2131) in HL-60 cell lysate. PI4K2A (arrow) was detected using purified Polyclonal antibody.
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PI4K2A polyclonal antibody (Cat # PAB2131) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Immunofluorescence
A : Negative control of HeLa cells without Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence.
B : Immunofluorescence analysis of PI4K2A polyclonal antibody (Cat # PAB2131) in HeLa cells.
Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PI4K2A.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PI4K2A.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
Immunofluorescence (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of PI4K2A polyclonal antibody (Cat # PAB2131) in HL-60 cell lysate. PI4K2A (arrow) was detected using purified Polyclonal antibody.
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PI4K2A polyclonal antibody (Cat # PAB2131) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.Immunofluorescence
A : Negative control of HeLa cells without Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence.
B : Immunofluorescence analysis of PI4K2A polyclonal antibody (Cat # PAB2131) in HeLa cells.
Primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). -
Gene Info — PI4K2A
Entrez GeneID
55361Protein Accession#
NP_060895;Q9BTU6Gene Name
PI4K2A
Gene Alias
DKFZp761G1923, PI4KII, PIK42A, RP11-548K23.6
Gene Description
phosphatidylinositol 4-kinase type 2 alpha
Omim ID
609763Gene Ontology
HyperlinkGene Summary
Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).[supplied by OMIM
Other Designations
OTTHUMP00000020224|phosphatidylinositol 4-kinase type II (PI4KII)
-
Interactome
-
Disease
-
Publication Reference
-
Phosphatidylinositol 4 phosphate regulates targeting of clathrin adaptor AP-1 complexes to the Golgi.
Wang YJ, Wang J, Sun HQ, Martinez M, Sun YX, Macia E, Kirchhausen T, Albanesi JP, Roth MG, Yin HL.
Cell 2003 Aug; 114(3):299.
Application：IF, WB, Human, CV1, HeLa cells.
-
Cloning of a human type II phosphatidylinositol 4-kinase reveals a novel lipid kinase family.
Minogue S, Anderson JS, Waugh MG, dos Santos M, Corless S, Cramer R, Hsuan JJ.
J Biol Chem 2001 Feb; 276(20).
-
A novel family of phosphatidylinositol 4-kinases conserved from yeast to humans.
Barylko B, Gerber SH, Binns DD, Grichine N, Khvotchev M, Sudhof TC, Albanesi JP.
The Journal of Biological Chemistry 2001 Jan; 276(11):7705.
-
Phosphatidylinositol 4 phosphate regulates targeting of clathrin adaptor AP-1 complexes to the Golgi.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com