C9orf80 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with C9orf80 polyclonal antibody (Cat # PAB21289) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human adrenal gland with C9orf80 polyclonal antibody (Cat # PAB21289) shows strong nuclear positivity in cortical cells at 1:20-1:50 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with C9orf80 polyclonal antibody (Cat # PAB21289) at 1-4 ug/mL dilution shows positivity in nuclei but not nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant C9orf80.
Immunogen
Recombinant protein corresponding to amino acids of human C9orf80.
Sequence
KEKRKLLMQNQSSTNHPGASIALSRPSLNKDFRDHAEQQHIAAQQKAALQHAHAHSSGYFITQDSAFGNL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with C9orf80 polyclonal antibody (Cat # PAB21289) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human adrenal gland with C9orf80 polyclonal antibody (Cat # PAB21289) shows strong nuclear positivity in cortical cells at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with C9orf80 polyclonal antibody (Cat # PAB21289) at 1-4 ug/mL dilution shows positivity in nuclei but not nucleoli. -
Gene Info — C9orf80
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Interactome
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