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Last updated: 2017/3/26
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PIK3CA polyclonal antibody

  • Catalog # : PAB2126
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PIK3CA.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to internal region of human PIK3CA.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of PIK3CA polyclonal antibody (Cat # PAB2126) in K-562 cell line lysates (35 ug/lane). PIK3CA (arrow) was detected using the purified Polyclonal antibody.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of PIK3CA (arrow) using PIK3CA polyclonal antibody (Cat # PAB2126) in 293 cell line lysates transiently transfected with the PIK3CA gene (1 ug/lane). PIK3CA (arrow) was detected using the purified Polyclonal antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PIK3CA polyclonal antibody (Cat # PAB2126) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
    This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5290
  • Protein Accession#:
  • NP_006209;Q14CW1
  • Gene Name:
  • PIK3CA
  • Gene Alias:
  • MGC142161,MGC142163,PI3K,p110-alpha
  • Gene Description:
  • phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • Gene Summary:
  • Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq
  • Other Designations:
  • PI3-kinase p110 subunit alpha,PtdIns-3-kinase p110,phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha,phosphatidylinositol 3-kinase, catalytic, alpha polypeptide,phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
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