PPM1L polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with PPM1L polyclonal antibody (Cat # PAB21258) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human skeletal muscle with PPM1L polyclonal antibody (Cat # PAB21258) shows heterogeneous cytoplasmic positivity in myocytes at 1:20-1:50 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant PPM1L.
Immunogen
Recombinant protein corresponding to amino acids of human PPM1L.
Sequence
DLDKLQPEFMILASDGLWDAFSNEEAVRFIKERLDEPHFGAKSIVLQSFYRGCPDNITVMVVKFRNSSK
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with PPM1L polyclonal antibody (Cat # PAB21258) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human skeletal muscle with PPM1L polyclonal antibody (Cat # PAB21258) shows heterogeneous cytoplasmic positivity in myocytes at 1:20-1:50 dilution. -
Gene Info — PPM1L
Entrez GeneID
151742Protein Accession#
Q5SGD2Gene Name
PPM1L
Gene Alias
MGC132545, MGC132547, PP2C-epsilon, PP2CE, PPM1-LIKE
Gene Description
protein phosphatase 1 (formerly 2C)-like
Gene Ontology
HyperlinkGene Summary
PPM1L, or PP2CE, belongs to the PP2C group of serine/threonine phosphatases, which are distinguished from other phosphatases by their structure, absolute requirement for Mg(2+) or Mn(2+), and insensitivity to okadaic acid. PP2Cs regulate stress-activated protein kinase (SAPK; see MIM 601158) signaling cascades that respond to extracellular stimuli (Jin et al., 2004 [PubMed 15560375]).[supplied by OMIM
Other Designations
PP2C epsilon|protein phosphatase 2C epsilon
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Interactome
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Disease
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