NAPEPLD polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant NAPEPLD.
Immunogen
Recombinant protein corresponding to amino acids of human NAPEPLD.
Sequence
MKYQHVDPEEAVRIHTDVQTKKSMAIHWGTFALANEHYLEPPVKLNEALERYGLNAEDFFVLKHGESRYLNNDDE
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with NAPEPLD polyclonal antibody (Cat # PAB21247) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with NAPEPLD polyclonal antibody (Cat # PAB21247) shows strong cytoplasmic positivity in tubular cells at 1:50-1:200 dilution. -
Gene Info — NAPEPLD
Entrez GeneID
222236Protein Accession#
Q6IQ20Gene Name
NAPEPLD
Gene Alias
DKFZp781D1098, FMP30, NAPE-PLD
Gene Description
N-acyl phosphatidylethanolamine phospholipase D
Gene Ontology
HyperlinkGene Summary
NAPEPLD is a phospholipase D type enzyme that catalyzes the release of N-acylethanolamine (NAE) from N-acyl-phosphatidylethanolamine (NAPE) in the second step of the biosynthesis of N-acylethanolamine (Okamoto et al., 2004 [PubMed 14634025]).[supplied by OMIM
Other Designations
N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D
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