NLE1 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against recombinant NLE1.
Immunogen
Recombinant protein corresponding to amino acids of human NLE1.
Sequence
TIKVWRAHDGVLCRTLQGHGHWVNTMALSTDYALRTGAFEPAEASVNPQDLQGSLQELKERALSRYNLVRGQGPERLVSGSDDFTL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with NLE1 polyclonal antibody (Cat # PAB21176) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human oral mucosa with NLE1 polyclonal antibody (Cat # PAB21176) shows strong nucleolar positivity in squamous epithelial cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with NLE1 polyclonal antibody (Cat # PAB21176) at 1-4 ug/mL dilution shows positivity in nucleus and nucleoli. -
Gene Info — NLE1
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Interactomes
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