POLDIP3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant POLDIP3.
Immunogen
Recombinant protein corresponding to amino acids of human POLDIP3.
Sequence
SLDELIRKRGAAAKGRLNARPGVGGVRSRVGIQQGLLSQSTRTATFQQRFDARQKIGLSDARLKLGVKDAREKLLQKDARFRIKGKVQDAR
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with POLDIP3 polyclonal antibody (Cat # PAB21164) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with POLDIP3 polyclonal antibody (Cat # PAB21164) shows strong nuclear positivity in cells in seminiferus ducts at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with POLDIP3 polyclonal antibody (Cat # PAB21164) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and vesicles. -
Gene Info — POLDIP3
Entrez GeneID
84271Protein Accession#
Q9BY77Gene Name
POLDIP3
Gene Alias
KIAA1649, PDIP46, SKAR
Gene Description
polymerase (DNA-directed), delta interacting protein 3
Omim ID
611520Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit. This protein is a specific target of S6 kinase 1 and regulates cell growth. Two transcript variants that encode different protein isoforms have been identified. [provided by RefSeq
Other Designations
DNA polymerase delta interacting protein 3|OTTHUMP00000028521|RNA-binding protein P46|S6K1 Aly/REF-like target|polymerase delta interacting protein 46
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