PAK1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PAK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding T423 of human PAK1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of PAK1 (arrow) using PAK1 polyclonal antibody (Cat # PAB2110). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK1 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PAK1 polyclonal antibody (Cat # PAB2110) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — PAK1
Entrez GeneID
5058Protein Accession#
NP_002567;Q13153Gene Name
PAK1
Gene Alias
MGC130000, MGC130001, PAKalpha
Gene Description
p21 protein (Cdc42/Rac)-activated kinase 1
Omim ID
602590Gene Ontology
HyperlinkGene Summary
PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
STE20 homolog, yeast|p21-activated kinase 1|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)|p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
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Publication Reference
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The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis.
Chen S, Yin X, Zhu X, Yan J, Ji S, Chen C, Cai M, Zhang S, Zong H, Hu Y, Yuan Z, Shen Z, Gu J.
The Journal of Biological Chemistry 2003 May; 278(22):20029.
Application:IP, KA, WB-Tr, Mouse, NIH/3T3 cells.
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Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells.
Sells MA, Knaus UG, Bagrodia S, Ambrose DM, Bokoch GM, Chernoff J.
Current Biology 1997 Mar; 7(3):202.
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Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway.
Brown JL, Stowers L, Baer M, Trejo J, Coughlin S, Chant J.
Current Biology 1996 May; 6(5):598.
Application:WB-Tr, Monkey, COS-7 cells.
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The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis.
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