FAM174A polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with FAM174A polyclonal antibody (Cat # PAB21070) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human hippocampus with FAM174A polyclonal antibody (Cat # PAB21070) shows strong cytoplasmic positivity with a granular pattern in neuronal cells at 1:50-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant FAM174A.
Immunogen
Recombinant protein corresponding to amino acids of human FAM174A.
Sequence
RRNRKTRRYGVLDTNIENMELTPLEQDDEDDDNTLFDANHPRR
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with FAM174A polyclonal antibody (Cat # PAB21070) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human hippocampus with FAM174A polyclonal antibody (Cat # PAB21070) shows strong cytoplasmic positivity with a granular pattern in neuronal cells at 1:50-1:200 dilution. -
Gene Info — FAM174A
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Interactome
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