LEMD2 polyclonal antibody
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human skin with LEMD2 polyclonal antibody (Cat # PAB20940) shows nuclear membranous positivity in epidermal cells at 1:10-1:20 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with LEMD2 polyclonal antibody (Cat # PAB20940) at 1-4 ug/mL dilution shows positivity in nuclear membrane.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant LEMD2.
Immunogen
Recombinant protein corresponding to amino acids of human LEMD2.
Sequence
EDNMKLLPVDCERKTDEFCQAKQKAALLELLHELYNFLAIQAGNFECGNPENLKSKCIPVMEAQEYIANVTSSSSAKFEAALTWILSSNKDVGIWLKGEDQSELVTTVDKVVCLESAHP
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:10-1:20)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human skin with LEMD2 polyclonal antibody (Cat # PAB20940) shows nuclear membranous positivity in epidermal cells at 1:10-1:20 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with LEMD2 polyclonal antibody (Cat # PAB20940) at 1-4 ug/mL dilution shows positivity in nuclear membrane. -
Gene Info — LEMD2
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Interactome
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Disease
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Publication Reference
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Unrestrained ESCRT-III Drives Micronuclear Catastrophe and Chromosome Fragmentation.
Marina Vietri, Sebastian W Schultz, Aurélie Bellanger, Carl M Jones, Louise I Petersen, Camilla Raiborg, Ellen Skarpen, Christeen Ramane J Pedurupillay, Ingrid Kjos, Eline Kip, Romy Timmer, Ashish Jain, Philippe Collas, Roland L Knorr, Sushma N Grellscheid, Halim Kusumaatmaja, Andreas Brech, Francesca Micci, Harald Stenmark, Coen Campsteijn.
Nature Cell Biology 2020 Jul; 22(7):856.
Application：WB-Ce, Human, HeLa cells.
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Unrestrained ESCRT-III Drives Micronuclear Catastrophe and Chromosome Fragmentation.
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