HADHA polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of cell lysates with HADHA polyclonal antibody (Cat # PAB20813) at 1:250-1:500 dilution.
Lane 1 : NIH/3T3
Lane 2 : NBT-II
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with HADHA polyclonal antibody (Cat # PAB20813) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human small intestine with HADHA polyclonal antibody (Cat # PAB20813) shows strong cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with HADHA polyclonal antibody (Cat # PAB20813) at 1-4 ug/mL dilution shows positivity in mitochondria.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant HADHA.
Immunogen
Recombinant protein corresponding to amino acids of human HADHA.
Sequence
IEYLEEVAITFAKGLADKKISPKRDKGLVEKLTAYAMTIPFVRQQVYKKVEEKVRKQTKGLYPAPLKIIDVVKTGIEQGSDAGYLCESQKFGELVMTKESKALMGLYHGQVLCKKNKFGAPQKDVKHLAILGAGLMGAGIAQVSVDK
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of cell lysates with HADHA polyclonal antibody (Cat # PAB20813) at 1:250-1:500 dilution.
Lane 1 : NIH/3T3
Lane 2 : NBT-IIWestern Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with HADHA polyclonal antibody (Cat # PAB20813) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human small intestine with HADHA polyclonal antibody (Cat # PAB20813) shows strong cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with HADHA polyclonal antibody (Cat # PAB20813) at 1-4 ug/mL dilution shows positivity in mitochondria. -
Gene Info — HADHA
Entrez GeneID
3030Protein Accession#
P40939Gene Name
HADHA
Gene Alias
ECHA, GBP, HADH, LCHAD, MGC1728, MTPA, TP-ALPHA
Gene Description
hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
Omim ID
600890Gene Ontology
HyperlinkGene Summary
This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq
Other Designations
3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit|3-oxoacyl-CoA thiolase|78 kDa gastrin-binding protein|OTTHUMP00000122457|mitochondrial long-chain 2-enoyl-Coenzyme A (CoA) hydratase, alpha subunit|mitochondrial long-chain L-3-hydroxyacyl-Coenzyme A (Co
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Interactome
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Pathway
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Disease
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