GPRC5B polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with GPRC5B polyclonal antibody (Cat # PAB20805) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with GPRC5B polyclonal antibody (Cat # PAB20805) shows membranous positivity in glomeruli at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with GPRC5B polyclonal antibody (Cat # PAB20805) at 1-4 ug/mL dilution shows positivity in vesicles.
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Specifications
Product Description
Rabbit polyclonal antibody raised against recombinant GPRC5B.
Immunogen
Recombinant protein corresponding to amino acids of human GPRC5B.
Sequence
IHCTLLPALQENTPNYFDTSQPRMRETAFEEDVQLPRAYMENKAFSMDEHNAALRTAGFPNGSLGKRPSGSLGKRPSAPFRSNVYQPTEMAVVLNGGTIPTAP
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with GPRC5B polyclonal antibody (Cat # PAB20805) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with GPRC5B polyclonal antibody (Cat # PAB20805) shows membranous positivity in glomeruli at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with GPRC5B polyclonal antibody (Cat # PAB20805) at 1-4 ug/mL dilution shows positivity in vesicles. -
Gene Info — GPRC5B
Entrez GeneID
51704Protein Accession#
Q9NZH0Gene Name
GPRC5B
Gene Alias
RAIG-2, RAIG2
Gene Description
G protein-coupled receptor, family C, group 5, member B
Omim ID
605948Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the type 3 G protein-coupled receptor family. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The specific function of this protein is unknown; however, this protein may mediate the cellular effects of retinoic acid on the G protein signal transduction cascade. [provided by RefSeq
Other Designations
G protein-coupled receptor, family C, group 1, member B|retinoic acid responsive gene protein
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Interactomes
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Diseases
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