DYRK1A polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of DYRK1A polyclonal antibody (Cat # PAB2067) in 293 cell line lysates (35 ug/lane). DYRK1A (arrow) was detected using the purified Polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinomareacted with DYRK1A polyclonal antibody (Cat # PAB2067), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DYRK1A.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human DYRK1A.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of DYRK1A polyclonal antibody (Cat # PAB2067) in 293 cell line lysates (35 ug/lane). DYRK1A (arrow) was detected using the purified Polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinomareacted with DYRK1A polyclonal antibody (Cat # PAB2067), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — DYRK1A
Entrez GeneID
1859Protein Accession#
NP_001387;Q13627Gene Name
DYRK1A
Gene Alias
DYRK, DYRK1, HP86, MNB, MNBH
Gene Description
dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A
Omim ID
600855Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms. [provided by RefSeq
Other Designations
MNB/DYRK protein kinase|OTTHUMP00000109090|dual specificity YAK1-related kinase|minibrain homolog|mnb protein kinase homolog hp86|protein kinase minibrain homolog|serine/threonine kinase MNB|serine/threonine-specific protein kinase
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Interactome
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Disease
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Publication Reference
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Increased expression of Dyrk1a in HPV16 immortalized keratinocytes enable evasion of apoptosis.
Chang HS, Lin CH, Yang CH, Yen MS, Lai CR, Chen YR, Liang YJ, Yu WC.
International Journal of Cancer 2007 Jun; 120(11):2377.
Application：IF, IHC-Fr, WB-Tr, Human, FK 30 cells, Normal and malignant exocervical tissue specimens.
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Dual-specificity tyrosine phosphorylation-regulated kinase 1A does not require tyrosine phosphorylation for activity in vitro.
Adayev T, Chen-Hwang MC, Murakami N, Lee E, Bolton DC, Hwang YW.
Biochemistry 2007 Jun; 46(25):7614.
Application：WB, Cercopithecus aethiops, COS7 cells.
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DYRK1A autophosphorylation on serine residue 520 modulates its kinase activity via 14-3-3 binding.
Alvarez M, Altafaj X, Aranda S, de la Luna S.
Molecular Biology of the Cell 2007 Apr; 18(4):1167.
Application：IP, WB, Human, Rat, PC-12, U2-OS cells.
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Increased expression of Dyrk1a in HPV16 immortalized keratinocytes enable evasion of apoptosis.
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