PAWR polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant PAWR.
Immunogen
Recombinant protein corresponding to amino acids of human PAWR.
Sequence
LQEPPRTVSGRYKSTTSVSEEDVSSRYSRTDRSGFPRYNRDANVSGTLVSSSTLEKKIEDLEKEVVRERQENLRLVRLMQDKEEMIGKLKEEIDLLNRDLDDIEDENEQLKQENKT
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of cell lysates with PAWR polyclonal antibody (Cat # PAB20610) at 1:250-1:500 dilution.
Lane 1 : NIH/3T3
Lane 2 : NBT-IIWestern Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with PAWR polyclonal antibody (Cat # PAB20610) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with PAWR polyclonal antibody (Cat # PAB20610) shows strong cytoplasmic and nuclear positivity in neuronal cells and glial cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
Using PAWR polyclonal antibody (Cat # PAB20610). -
Gene Info — PAWR
Entrez GeneID
5074Protein Accession#
Q96IZ0Gene Name
PAWR
Gene Alias
PAR4, Par-4
Gene Description
PRKC, apoptosis, WT1, regulator
Omim ID
601936Gene Ontology
HyperlinkGene Summary
The tumor suppressor WT1 represses and activates transcription. The protein encoded by this gene is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells. [provided by RefSeq
Other Designations
WT1-interacting protein|prostate apoptosis response protein 4|prostate apoptosis response protein PAR-4|transcriptional repressor PAR4
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Interactome
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Disease
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