USP36 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant USP36.
Immunogen
Recombinant protein corresponding to amino acids of human USP36.
Sequence
DSLVHSSNVKVVLNQQAYVLFYLRIPGSKKSPEGLISRTGSSSLPGRPSVIPDHSKKNIGNGIISSPLTGKRQDSGTMKKPHTTEEIGVPISRNGSTLGLKSQNGCIPPKLPSGSPSPKLSQTPTHMPTILDDPGKKVKKPAPP
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with USP36 polyclonal antibody (Cat # PAB20583) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human liver with USP36 polyclonal antibody (Cat # PAB20583) shows strong nuclear positivity in hepatocytes at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with USP36 polyclonal antibody (Cat # PAB20583) at 1-4 ug/mL dilution shows positivity in nucleoli. -
Gene Info — USP36
Entrez GeneID
57602Protein Accession#
Q9P275Gene Name
USP36
Gene Alias
DUB1
Gene Description
ubiquitin specific peptidase 36
Gene Ontology
HyperlinkGene Summary
Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP36 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]).[supplied by OMIM
Other Designations
deubiquitinating enzyme 1|ubiquitin specific protease 36
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