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Last updated: 2017/11/19
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EN1 polyclonal antibody

  • Catalog # : PAB2058
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of EN1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human EN1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of EN1 polyclonal antibody (Cat # PAB2058) in NCI-H460 cell line lysates (35 ug/lane). EN1 (arrow) was detected using the purified Polyclonal antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 polyclonal antibody (Cat # PAB2058) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
    This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2019
  • Protein Accession#:
  • NP_001417;Q05925
  • Gene Name:
  • EN1
  • Gene Alias:
  • -
  • Gene Description:
  • engrailed homeobox 1
  • Gene Summary:
  • Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000162091,engrailed homolog 1
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