TMEM109 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant TMEM109.
Immunogen
Recombinant protein corresponding to amino acids of human TMEM109.
Sequence
LEAKVRGLERQVEELRWRQRRAAKGARSVEE
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:500-1:1000)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of cell lysates with TMEM109 polyclonal antibody (Cat # PAB20560) at 1:250-1:500 dilution.
Lane 1 : NIH/3T3
Lane 2 : NBT-IIWestern Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with TMEM109 polyclonal antibody (Cat # PAB20560) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human pancreas with TMEM109 polyclonal antibody (Cat # PAB20560) shows strong cytoplasmic positivity in exocrine glandular cells at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with TMEM109 polyclonal antibody (Cat # PAB20560) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, nuclear membrane, cytoplasm. -
Gene Info — TMEM109
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