RICTOR polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of RICTOR polyclonal antibody (Cat # PAB2054) in SK-BR-3 cell line lysates (35 ug/lane).RICTOR (arrow) was detected using the purified Polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testis tissue reacted with RICTOR polyclonal antibody (Cat # PAB2054) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RICTOR.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human RICTOR.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemsitry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of RICTOR polyclonal antibody (Cat # PAB2054) in SK-BR-3 cell line lysates (35 ug/lane).RICTOR (arrow) was detected using the purified Polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testis tissue reacted with RICTOR polyclonal antibody (Cat # PAB2054) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — RICTOR
Entrez GeneID
253260Protein Accession#
NP_689969;Q6R327Gene Name
RICTOR
Gene Alias
DKFZp686B11164, KIAA1999, MGC39830, mAVO3
Gene Description
rapamycin-insensitive companion of mTOR
Omim ID
609022Gene Ontology
HyperlinkGene Summary
RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM
Other Designations
TORC2-specific protein AVO3|pianissimo
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of Protor as a novel Rictor-binding component of mTOR complex-2.
Pearce LR, Huang X, Boudeau J, Pawlowski R, Wullschleger S, Deak M, Ibrahim AF, Gourlay R, Magnuson MA, Alessi DR.
The Biochemical Journal 2007 Aug; 405(3):513.
Application：IP, WB-Tr, Human, HEK 293 cells.
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Identification of Sin1 as an essential TORC2 component required for complex formation and kinase activity.
Yang Q, Inoki K, Ikenoue T, Guan KL.
Genes & Development 2006 Oct; 20(20):2820.
Application：IF, IP-WB, WB-Tr, Human, HeLa, HEK293 cells.
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SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity.
Jacinto E, Facchinetti V, Liu D, Soto N, Wei S, Jung SY, Huang Q, Qin J, Su B.
Cell 2006 Sep; 127(1):125.
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Identification of Protor as a novel Rictor-binding component of mTOR complex-2.
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