MANEA polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human gallbladder with MANEA polyclonal antibody (Cat # PAB20528) shows moderate cytoplasmic positivity, with a granular pattern, in glandular cells.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with MANEA polyclonal antibody (Cat # PAB20528) at 1-4 ug/mL dilution shows positivity in golgi.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant MANEA.
Immunogen
Recombinant protein corresponding to amino acids of human MANEA.
Sequence
LRPNTATFGAPFGLDLLPELHQRTIHLGKNFDFQKSDRINSETNTKNLKSVEITMKPSKASELNLDELPPLNNYLHVFYYSWYGNPQFDGKYIHWNHPVLEHWDPRIAKNYPQGRHNPPDDIGSSFYPELGSYSSRD
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human gallbladder with MANEA polyclonal antibody (Cat # PAB20528) shows moderate cytoplasmic positivity, with a granular pattern, in glandular cells.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with MANEA polyclonal antibody (Cat # PAB20528) at 1-4 ug/mL dilution shows positivity in golgi. -
Gene Info — MANEA
Entrez GeneID
79694Protein Accession#
Q5SRI9Gene Name
MANEA
Gene Alias
DKFZp686D20120, ENDO, FLJ12838, hEndo
Gene Description
mannosidase, endo-alpha
Gene Ontology
HyperlinkGene Summary
N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM
Other Designations
OTTHUMP00000017907|alpha 1,2-endomannosidase|mandaselin
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Interactome
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Disease
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Publication Reference
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Modeling Glycan Processing Reveals Golgi-Enzyme Homeostasis upon Trafficking Defects and Cellular Differentiation.
Fisher P, Spencer H, Thomas-Oates J, Wood AJ, Ungar D.
Cell Reports 2019 Apr; 27(4):1231.
Application：IF, Human, HEK 293T cells.
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Modeling Glycan Processing Reveals Golgi-Enzyme Homeostasis upon Trafficking Defects and Cellular Differentiation.
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