DCLK1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The DCLK1 polyclonal antibody (Cat # PAB2050) is used in Western blot to detect DCLK1 in mouse heart tissue lysate.
Western Blot (Cell lysate)
Western blot analysis of ZR-75-1 cell lysate (35 ug/lane) with DCLK1 polyclonal antibody (Cat # PAB2050).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with DCLK1 polyclonal antibody (Cat # PAB2050), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunohistochemistry
Expression pattern of doublecortin and DCLK1 in normal mouse small intestine.
A : Immunohistochemical staining of normal small intestine for DCLK1. Arrows indicate the cells positive for DCLK1 in the stem cell zone.
B : Preincubation with blocking peptide completely abolished DCLK1 immunoreactivity.
C : Immunohistochemical staining of normal small intestine for DCLK1. Brown indicates the cells positive for DCLK1 (indicated by the arrows).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DCLK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 690-720 at C-terminus of human DCLK1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The DCLK1 polyclonal antibody (Cat # PAB2050) is used in Western blot to detect DCLK1 in mouse heart tissue lysate.Western Blot (Cell lysate)
Western blot analysis of ZR-75-1 cell lysate (35 ug/lane) with DCLK1 polyclonal antibody (Cat # PAB2050).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with DCLK1 polyclonal antibody (Cat # PAB2050), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Immunohistochemistry
Expression pattern of doublecortin and DCLK1 in normal mouse small intestine.
A : Immunohistochemical staining of normal small intestine for DCLK1. Arrows indicate the cells positive for DCLK1 in the stem cell zone.
B : Preincubation with blocking peptide completely abolished DCLK1 immunoreactivity.
C : Immunohistochemical staining of normal small intestine for DCLK1. Brown indicates the cells positive for DCLK1 (indicated by the arrows). -
Gene Info — DCLK1
Entrez GeneID
9201Protein Accession#
NP_001317000Gene Name
DCLK1
Gene Alias
DCAMKL1, DCDC3A, DCLK, KIAA0369
Gene Description
doublecortin-like kinase 1
Omim ID
604742Gene Ontology
HyperlinkGene Summary
DCAMKL1 is a microtubule-associated protein that phosphorylates itself and myelin basic protein (MBP; MIM 159430). DCAMKL1 has microtubule polymerizing activity that is independent of its protein kinase activity (Lin et al., 2000 [PubMed 11124993]).[supplied by OMIM
Other Designations
OTTHUMP00000018250|doublecortin and CaM kinase-like 1
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Interactome
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Disease
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Publication Reference
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Identification of a novel putative gastrointestinal stem cell and adenoma stem cell marker, doublecortin and CaM kinase-like-1, following radiation injury and in adenomatous polyposis coli/multiple intestinal neoplasia mice.
May R, Riehl TE, Hunt C, Sureban SM, Anant S, Houchen CW.
Stem Cells 2007 Nov; 26(3):630.
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Identification of a novel putative gastrointestinal stem cell and adenoma stem cell marker, doublecortin and CaM kinase-like-1, following radiation injury and in adenomatous polyposis coli/multiple intestinal neoplasia mice.
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