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Last updated: 2017/2/19
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DCLK1 polyclonal antibody

  • Catalog # : PAB2050
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of DCLK1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 690-720 at C-terminus of human DCLK1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • The DCLK1 polyclonal antibody (Cat # PAB2050) is used in Western blot to detect DCLK1 in mouse heart tissue lysate.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of ZR-75-1 cell lysate (35 ug/lane) with DCLK1 polyclonal antibody (Cat # PAB2050).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with DCLK1 polyclonal antibody (Cat # PAB2050), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Immunohistochemistry
  • Immunohistochemistry
  • Expression pattern of doublecortin and DCLK1 in normal mouse small intestine.
    A : Immunohistochemical staining of normal small intestine for DCLK1. Arrows indicate the cells positive for DCLK1 in the stem cell zone.
    B : Preincubation with blocking peptide completely abolished DCLK1 immunoreactivity.
    C : Immunohistochemical staining of normal small intestine for DCLK1. Brown indicates the cells positive for DCLK1 (indicated by the arrows).
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 9201
  • Gene Name:
  • DCLK1
  • Gene Alias:
  • DCAMKL1,DCDC3A,DCLK,KIAA0369
  • Gene Description:
  • doublecortin-like kinase 1
  • Gene Summary:
  • DCAMKL1 is a microtubule-associated protein that phosphorylates itself and myelin basic protein (MBP; MIM 159430). DCAMKL1 has microtubule polymerizing activity that is independent of its protein kinase activity (Lin et al., 2000 [PubMed 11124993]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000018250,doublecortin and CaM kinase-like 1
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