FUS polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of cell lysates with FUS polyclonal antibody (Cat # PAB20448) at 1:250-1:500 dilution.
Lane 1 : NIH/3T3
Lane 2 : NBT-II
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with FUS polyclonal antibody (Cat # PAB20448) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon with FUS polyclonal antibody (Cat # PAB20448) shows strong nuclear positivity in glandular cells at 1:1000-1:2500 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with FUS polyclonal antibody (Cat # PAB20448) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant FUS.
Immunogen
Recombinant protein corresponding to amino acids of human FUS.
Sequence
SSQSSYGQQSSYPGYGQQPAPSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNSSSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDR
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:1000-1:2500)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of cell lysates with FUS polyclonal antibody (Cat # PAB20448) at 1:250-1:500 dilution.
Lane 1 : NIH/3T3
Lane 2 : NBT-IIWestern Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with FUS polyclonal antibody (Cat # PAB20448) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon with FUS polyclonal antibody (Cat # PAB20448) shows strong nuclear positivity in glandular cells at 1:1000-1:2500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with FUS polyclonal antibody (Cat # PAB20448) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli. -
Gene Info — FUS
Entrez GeneID
2521Protein Accession#
P35637Gene Name
FUS
Gene Alias
CHOP, FUS-CHOP, FUS1, TLS, TLS/CHOP, hnRNP-P2
Gene Description
fusion (involved in t(12;16) in malignant liposarcoma)
Omim ID
137070Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq
Other Designations
FUS-CHOP fusion protein|FUS-CHOP protein fusion|Fusion gene in myxoid liposarcoma|fus-like protein|fus/tls-chop oncogene|heterogeneous nuclear ribonucleoprotein P2|translocated in liposarcoma
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Interactome
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Disease
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Publication Reference
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RNA and the RNA-binding protein FUS act in concert to prevent TDP-43 spatial segregation.
Clément Demongin, Samuel Tranier, Vandana Joshi, Léa Ceschi, Bénédicte Desforges, David Pastré, Loic Hamon.
The Journal of Biological Chemistry 2024 Feb; 300(3):105716.
Application：Proximity Ligation Assay , Human, Hela cells.
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Therapeutic modulation of GSTO activity rescues FUS-associated neurotoxicity via deglutathionylation in ALS disease models.
Sun Joo Cha, Seongsoo Lee, Hyun-Jun Choi, Yeo Jeong Han, Yu-Mi Jeon, Myungjin Jo, Shinrye Lee, Minyeop Nahm, Su Min Lim, Seung Hyun Kim, Hyung-Jun Kim, Kiyoung Kim.
Developmental Cell 2022 Mar; 57(6):783.
Application：IF, Fruit fly, Fruit fly brain.
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RNA and the RNA-binding protein FUS act in concert to prevent TDP-43 spatial segregation.
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