SEPHS2 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SEPHS2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human SEPHS2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of SEPHS2 polyclonal antibody (Cat # PAB2041) in HepG2 cell line lysate (35 ug/lane). SEPHS2 (arrow) was detected using the purified Polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with SEPHS2 polyclonal antibody (Cat # PAB2041) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — SEPHS2
Entrez GeneID
22928Protein Accession#
NP_036380;Q99611Gene Name
SEPHS2
Gene Alias
SPS2
Gene Description
selenophosphate synthetase 2
Omim ID
606218Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem-loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop. Alternatively spliced transcripts have been identified, but their biological validity has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000045871|selenide,water dikinase 2|selenium donor protein 2|selenophosphate synthase
-
Interactome
-
Pathway
-
Publication Reference
-
Selenophosphate synthetase genes from lung adenocarcinoma cells: Sps1 for recycling L-selenocysteine and Sps2 for selenite assimilation.
Tamura T, Yamamoto S, Takahata M, Sakaguchi H, Tanaka H, Stadtman TC, Inagaki K.
PNAS 2004 Nov; 101(46):16162.
-
Novel selenoproteins identified in silico and in vivo by using a conserved RNA structural motif.
Lescure A, Gautheret D, Carbon P, Krol A.
The Journal of Biological Chemistry 1999 Dec; 274(53):38147.
-
Identification of a novel selD homolog from eukaryotes, bacteria, and archaea: is there an autoregulatory mechanism in selenocysteine metabolism?
Guimaraes MJ, Peterson D, Vicari A, Cocks BG, Copeland NG, Gilbert DJ, Jenkins NA, Ferrick DA, Kastelein RA, Bazan JF, Zlotnik A.
PNAS 1996 Dec; 93(26):15086.
Application:IP, WB-Tr, Monkey, COS-7 cells.
-
Selenophosphate synthetase genes from lung adenocarcinoma cells: Sps1 for recycling L-selenocysteine and Sps2 for selenite assimilation.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com