CALB2 polyclonal antibody
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More Files
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Specifications
Product Description
Rabbit polyclonal antibody raised against recombinant CALB2.
Immunogen
Recombinant protein corresponding to amino acids of human CALB2.
Sequence
MAGPQQQPPYLHLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQHVGSSAEFMEAWRKY
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of cell lysates with CALB2 polyclonal antibody (Cat # PAB20373) at 1:250-1:500 dilution.
Lane 1 : NIH/3T3
Lane 2 : NBT-IIWestern Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with CALB2 polyclonal antibody (Cat # PAB20373) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebellum with CALB2 polyclonal antibody (Cat # PAB20373) shows strong nuclear and cytoplasmic positivity in Purkinje cells and strong nuclear in cells in molecular layer at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with CALB2 polyclonal antibody (Cat # PAB20373) at 1-4 ug/mL dilution shows positivity in cytoplasm. -
Gene Info — CALB2
Entrez GeneID
794Protein Accession#
P22676Gene Name
CALB2
Gene Alias
CAL2
Gene Description
calbindin 2
Omim ID
114051Gene Ontology
HyperlinkGene Summary
This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. Three alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq
Other Designations
calbindin 2, (29kD, calretinin)|calbindin D29K|calretinin
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