RDBP polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with RDBP polyclonal antibody (Cat # PAB20363) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with RDBP polyclonal antibody (Cat # PAB20363) shows strong nuclear positivity in neuronal cells at 1:200-1:500 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with RDBP polyclonal antibody (Cat # PAB20363) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, mitochondria.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant RDBP.
Immunogen
Recombinant protein corresponding to amino acids of human RDBP.
Sequence
LLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESFVSSSDRLRELGPDG
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with RDBP polyclonal antibody (Cat # PAB20363) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with RDBP polyclonal antibody (Cat # PAB20363) shows strong nuclear positivity in neuronal cells at 1:200-1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with RDBP polyclonal antibody (Cat # PAB20363) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, mitochondria. -
Gene Info — RDBP
Entrez GeneID
7936Protein Accession#
P18615Gene Name
RDBP
Gene Alias
D6S45, NELF-E, RD, RDP
Gene Description
RD RNA binding protein
Omim ID
154040Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq
Other Designations
OTTHUMP00000029051|RD RNA-binding protein|major histocompatibility complex gene RD|negative elongation factor polypeptide E|nuclear protein
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Interactome
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Disease
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