PI4K2B polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant PI4K2B.
Immunogen
Recombinant protein corresponding to amino acids of human PI4K2B.
Sequence
LILPYISDMNFVQDLCEDLYELFKTDKGFDKATFESQMSVMRGQILNLTQALRDGKSPFQLVQIPCVIVERSQGGSQGRIVHLSNSFTQTVNCRKPF
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with PI4K2B polyclonal antibody (Cat # PAB20260) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon with PI4K2B polyclonal antibody (Cat # PAB20260) shows moderate cytoplasmic and membranous positivity in glandular cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with PI4K2B polyclonal antibody (Cat # PAB20260) at 1-4 ug/mL dilution shows positivity in cytoplasm. -
Gene Info — PI4K2B
Entrez GeneID
55300Protein Accession#
Q8TCG2Gene Name
PI4K2B
Gene Alias
FLJ11105, PI4KIIB, PIK42B
Gene Description
phosphatidylinositol 4-kinase type 2 beta
Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol 4-kinases (PI4Ks) phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate (PIP), an immediate precursor of several important signaling and scaffolding molecules. PIP itself may also have direct functional and structural roles. PI4K2B is a primarily cytosolic PI4K that is recruited to membranes, where it stimulates phosphatidylinositol 4,5-bisphosphate synthesis (Wei et al., 2002 [PubMed 12324459]).[supplied by OMIM
Other Designations
2610042N09Rik|4933409G22Rik|OTTHUMP00000158714|phosphatidylinositol 4-kinase type-II beta
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Interactome
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Disease
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