GIT1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant GIT1.
Immunogen
Recombinant protein corresponding to amino acids of human GIT1.
Sequence
HSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDS
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with GIT1 polyclonal antibody (Cat # PAB20257) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with GIT1 polyclonal antibody (Cat # PAB20257) shows strong cytoplasmic positivity in neuronal cells at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with GIT1 polyclonal antibody (Cat # PAB20257) at 1-4 ug/mL dilution shows positivity in cytoplasm, focal adhesions. -
Gene Info — GIT1
Entrez GeneID
28964Protein Accession#
Q9Y2X7Gene Name
GIT1
Gene Alias
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Gene Description
G protein-coupled receptor kinase interacting ArfGAP 1
Omim ID
608434Gene Ontology
HyperlinkOther Designations
G protein-coupled receptor kinase interactor 1|G protein-coupled receptor kinase-interactor 1|OTTHUMP00000163606
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Interactome
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Pathway
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Disease
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Publication Reference
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lncRNA miR4458HG modulates hepatocellular carcinoma progression by activating m6A-dependent glycolysis and promoting the polarization of tumor-associated macrophages.
Ying Ye, Menghan Wang, Guoyu Wang, Zhongchao Mai, Borong Zhou, Yang Han, Juhua Zhuang, Wei Xia.
Cellular and Molecular Life Sciences : CMLS 2023 Mar; 80(4):99.
Application:WB-Ce, Human, Human hepatocellular carcinoma.
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lncRNA miR4458HG modulates hepatocellular carcinoma progression by activating m6A-dependent glycolysis and promoting the polarization of tumor-associated macrophages.
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