MAD1L1 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with MAD1L1 polyclonal antibody (Cat # PAB20232) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with MAD1L1 polyclonal antibody (Cat # PAB20232) shows strong nuclear positivity in neuronal cells and glial cells at 1:200-1:500 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line A-431 with MAD1L1 polyclonal antibody (Cat # PAB20232) at 1-4 ug/mL dilution shows positivity in nucleus, cytoskeleton (actin filaments).
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant MAD1L1.
Immunogen
Recombinant protein corresponding to amino acids of human MAD1L1.
Sequence
TKVLHMSLNPTSVARQRLREDHSQLQAECERLRGLLRAMERGGTVPADLEAAAASLPSSKEVAELKKQVESAELKNQRLKEVFQTKIQEFRKACYTLTGYQIDITTENQYRLTSLYAEHPGDCLIFKATSPSGSKMQLLEIRRAPPLSNN
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with MAD1L1 polyclonal antibody (Cat # PAB20232) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with MAD1L1 polyclonal antibody (Cat # PAB20232) shows strong nuclear positivity in neuronal cells and glial cells at 1:200-1:500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with MAD1L1 polyclonal antibody (Cat # PAB20232) at 1-4 ug/mL dilution shows positivity in nucleus, cytoskeleton (actin filaments). -
Gene Info — MAD1L1
Entrez GeneID
8379Protein Accession#
Q9Y6D9Gene Name
MAD1L1
Gene Alias
HsMAD1, MAD1, PIG9, TP53I9, TXBP181
Gene Description
MAD1 mitotic arrest deficient-like 1 (yeast)
Gene Ontology
HyperlinkGene Summary
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
MAD1 (mitotic arrest deficient, yeast, homolog)-like 1|MAD1-like 1 protein|mitotic-arrest deficient 1, yeast, homolog-like 1|tumor protein p53 inducible protein 9
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Interactome
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Pathway
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Disease
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