RPS21 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of cell lysates with RPS21 polyclonal antibody (Cat # PAB20201) at 1:250-1:500 dilution.
Lane 1 : NIH/3T3
Lane 2 : NBT-II
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with RPS21 polyclonal antibody (Cat # PAB20201) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with RPS21 polyclonal antibody (Cat # PAB20201) shows strong cytoplasmic positivity in neuronal cells at 1:20-1:50 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with RPS21 polyclonal antibody (Cat # PAB20201) at 1-4 ug/mL dilution shows positivity in cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant RPS21.
Immunogen
Recombinant protein corresponding to amino acids of human RPS21.
Sequence
MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKADGIVSK
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of cell lysates with RPS21 polyclonal antibody (Cat # PAB20201) at 1:250-1:500 dilution.
Lane 1 : NIH/3T3
Lane 2 : NBT-IIWestern Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with RPS21 polyclonal antibody (Cat # PAB20201) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human cerebral cortex with RPS21 polyclonal antibody (Cat # PAB20201) shows strong cytoplasmic positivity in neuronal cells at 1:20-1:50 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG with RPS21 polyclonal antibody (Cat # PAB20201) at 1-4 ug/mL dilution shows positivity in cytoplasm. -
Gene Info — RPS21
Entrez GeneID
6227Protein Accession#
P63220Gene Name
RPS21
Gene Alias
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Gene Description
ribosomal protein S21
Omim ID
180477Gene Ontology
HyperlinkGene Summary
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S21E family of ribosomal proteins. It is located in the cytoplasm. Alternative splice variants that encode different protein isoforms have been described, but their existence has not been verified. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq
Other Designations
40S ribosomal protein S21|8.2 kDa differentiation factor|OTTHUMP00000031485
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Interactome
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Pathway
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