The DCK polyclonal antibody (Cat # PAB2020) is used in Western blot to detect DCK in mouse intestine tissue lysate.
Deoxycytidine kinase (DCK) immunoprecipitated from NCI-H460 cells with DCK polyclonal antibody (Cat # PAB2020) using the Pierce classic mammalian IP kit reagent as described as manufacturer instructions (lane 1, 3) and current protocols in Cell Biology, 1998, 7.2.1-7.2.21. Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with DCK polyclonal antibody (Cat # PAB2020). Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit). No specific reagents were employed to remove IgG from immunoprecipitated sample. Data courtesy of Dr. Stacy Shord, University of Illinois, Chicago.
Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq