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Last updated: 2016/12/4
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DCK polyclonal antibody

  • Catalog # : PAB2020
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of DCK.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DCK.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunoprecipitation (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • The DCK polyclonal antibody (Cat # PAB2020) is used in Western blot to detect DCK in mouse intestine tissue lysate.
  • Immunoprecipitation
  • Immunoprecipitation
  • Deoxycytidine kinase (DCK) immunoprecipitated from NCI-H460 cells with DCK polyclonal antibody (Cat # PAB2020) using the Pierce classic mammalian IP kit reagent as described as manufacturer instructions (lane 1, 3) and current protocols in Cell Biology, 1998, 7.2.1-7.2.21.
    Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with DCK polyclonal antibody (Cat # PAB2020).
    Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit).
    No specific reagents were employed to remove IgG from immunoprecipitated sample. Data courtesy of Dr. Stacy Shord, University of Illinois, Chicago.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1633
  • Protein Accession#:
  • NP_000779;P27707
  • Gene Name:
  • DCK
  • Gene Alias:
  • MGC117410,MGC138632
  • Gene Description:
  • deoxycytidine kinase
  • Gene Summary:
  • Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq
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