Immunohistochemistry (1:50-1:200) Western Blot (1:250-1:500) Immunofluorescence (1-4 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of cell lysates with ZNF146 polyclonal antibody (Cat # PAB20198) at 1:250-1:500 dilution. Lane 1 : NIH/3T3 Lane 2 : NBT-II
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Liver, Lane 4: Tonsil with ZNF146 polyclonal antibody (Cat # PAB20198) at 1:250-1:500 dilution.
Immunohistochemical staining of human breast with ZNF146 polyclonal antibody (Cat # PAB20198) shows strong nuclear positivity in glandular cells at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line A-431 with ZNF146 polyclonal antibody (Cat # PAB20198) at 1-4 ug/mL dilution shows positivity in nucleoli.