PRKAR1B polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of PRKAR1B polyclonal antibody (Cat # PAB2018) in mouse liver tissue lysate. PRKAR1B (arrow) was detected using purified Polyclonal antibody.
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKAR1B polyclonal antibody (Cat # PAB2018) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKAR1B.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PRKAR1B.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of PRKAR1B polyclonal antibody (Cat # PAB2018) in mouse liver tissue lysate. PRKAR1B (arrow) was detected using purified Polyclonal antibody.
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKAR1B polyclonal antibody (Cat # PAB2018) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — PRKAR1B
Entrez GeneID
5575Protein Accession#
NP_002726;P31321Gene Name
PRKAR1B
Gene Alias
PRKAR1
Gene Description
protein kinase, cAMP-dependent, regulatory, type I, beta
Omim ID
176911Gene Ontology
HyperlinkGene Summary
Cyclic AMP-dependent protein kinase A (PKA) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. The PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP.[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Publication Reference
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Human regulatory subunit RI beta of cAMP-dependent protein kinases: expression, holoenzyme formation and microinjection into living cells.
Solberg R, Tasken K, Wen W, Coghlan VM, Meinkoth JL, Scott JD, Jahnsen T, Taylor SS.
Experimental Cell Research 1994 Oct; 214(2):595.
Application：IP, WB-Ce, WB-Re, N/A, Recombinant protein.
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Molecular cloning, cDNA structure and tissue-specific expression of the human regulatory subunit RI beta of cAMP-dependent protein kinases.
Solberg R, Tasken K, Keiserud A, Jahnsen T.
Biochemical and Biophysical Research Communications 1991 Apr; 176(1):166.
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Human regulatory subunit RI beta of cAMP-dependent protein kinases: expression, holoenzyme formation and microinjection into living cells.
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