Products

Western Blot

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with UPF3B polyclonal antibody (Cat # PAB20093) at 1:250-1:500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical staining of human placenta with UPF3B polyclonal antibody (Cat # PAB20093) shows moderate cytoplasmic positivity in trophoblastic and decidual cells at 1:10-1:20 dilution.

Immunofluorescence

Immunofluorescent staining of human cell line U-2 OS with UPF3B polyclonal antibody (Cat # PAB20093) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Entrez GeneID

65109

Protein Accession#

Q9BZI7

Gene Name

UPF3B

Gene Alias

HUPF3B, MRXS14, RENT3B, UPF3X

Gene Description

UPF3 regulator of nonsense transcripts homolog B (yeast)

Omim ID

300298 300676

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq

Other Designations

OTTHUMP00000023923|OTTHUMP00000023924|UPF3 regulator of nonsense transcripts homolog B