TRMT5 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with TRMT5 polyclonal antibody (Cat # PAB20022) at 1:250-1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human stomach with TRMT5 polyclonal antibody (Cat # PAB20022) shows strong nuclear positivity in glandular cells at 1:200-1:500 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant TRMT5.
Immunogen
Recombinant protein corresponding to amino acids of human TRMT5.
Sequence
DGKDFLQGPVKEELMQLLGLSKERKPSVHVVMNLPAKAIEFLSAFKWLLDGQPCSSEFLPIVHCYSFSKDANPAEDVRQRAGAVLGISLEACSSVHLVRNVAPNKEMLCITFQIPASVLYKNQTRNPENHEDPPLKR
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200-1:500)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with TRMT5 polyclonal antibody (Cat # PAB20022) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human stomach with TRMT5 polyclonal antibody (Cat # PAB20022) shows strong nuclear positivity in glandular cells at 1:200-1:500 dilution. -
Gene Info — TRMT5
Entrez GeneID
57570Protein Accession#
Q32P41Gene Name
TRMT5
Gene Alias
KIAA1393, MGC111453, TRM5
Gene Description
TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae)
Omim ID
611023Gene Ontology
HyperlinkGene Summary
tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 [PubMed 15248782]).[supplied by OMIM
Other Designations
tRNA-(N1G37) methyltransferase
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Interactome
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Disease
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