ARFGAP3 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human pancreas with ARFGAP3 polyclonal antibody (Cat # PAB19985) shows moderate cytoplasmic positivity in exocrine glandular cells at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line A-431 with ARFGAP3 polyclonal antibody (Cat # PAB19985) at 1-4 ug/mL dilution shows positivity in cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant ARFGAP3.
Immunogen
Recombinant protein corresponding to amino acids of human ARFGAP3.
Sequence
SKQDILTIFKRLRSVPTNKVCFDCGAKNPSWASITYGVFLCIDCSGSHRSLGVHLSFIRSTELDSNWSWFQLRCMQVGGNASASSFFHQHGCSTNDTNAKYNSRAAQLYREKIKSLASQATRKHGTDLWLDSCVVPPLSPPP
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human pancreas with ARFGAP3 polyclonal antibody (Cat # PAB19985) shows moderate cytoplasmic positivity in exocrine glandular cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with ARFGAP3 polyclonal antibody (Cat # PAB19985) at 1-4 ug/mL dilution shows positivity in cytoplasm. -
Gene Info — ARFGAP3
Entrez GeneID
26286Protein Accession#
Q9NP61Gene Name
ARFGAP3
Gene Alias
ARFGAP1, FLJ45618
Gene Description
ADP-ribosylation factor GTPase activating protein 3
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq
Other Designations
ADP-ribosylation factor GTPase activating protein 1|OTTHUMP00000028526
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Interactome
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Pathway
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Disease
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