Rabbit polyclonal antibody raised against synthetic phosphopeptide of ATG13.
Synthetic phosphopeptide corresponding to residues surrounding S318 of human ATG13.
Theoretical MW (kDa):
The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human ATG13 pS318 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human ATG13 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with ATG13 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
ELISA (1:25000-1:175000) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of 293T with ATG13 (phospho S318) polyclonal antibody (Cat # PAB19948) at 1 ug/mL dilution. Lane 1: kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13; Lane 2: 93T Ulk1 + ATG13 lysate.