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Myb (phospho T486) polyclonal antibody

  • Catalog # : PAB19946
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of Myb.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T486 of mouse Myb.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 75
  • Reactivity:
  • Mouse
  • Specificity:
  • Reactivity occurs against mouse c-Myb pT486 protein and the antibody is specific for the phosphorylated form of the protein. This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells. Reactivity with non-phosphorylated mouse c-Myb is minimal by ELISA and western blot. A BLAST analysis was used to suggest reactivity with c-Myb from mouse based on a 100% homology with the immunizing sequence. Expect reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee; with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from human; and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (avian), both from cattle, all based on a 93% homology with the immunizing sequence. Cross-reactivity with c-Myb from other sources has not been determined.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Recommend Usage:
  • ELISA (1:5000-1:16000)
    Western Blot (1:2500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of COS7 cell untreated (Lane 1) or treated with okadaic acid (Lane 2) by using Myb (phospho T486) polyclonal antibody (Cat # PAB19946) at 1:2500 dilution.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • Myb
  • Gene Alias:
  • AI550390,M16449,MGC18531,c-myb
  • Gene Description:
  • myeloblastosis oncogene
  • Other Designations:
  • gag-myb protein,myeloblastosis proto-oncogene product,tumor-specific myb protein
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