Cytip polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis using Cytip polyclonal antibody (Cat # PAB19941) shows detection of endogenous Cybr from mouse splenocytes using anti-Cybr antibody to immunoprecipitate and western blot (lanes 1-3). Lane 1 shows reactivity of pre-immune sera. Lane 2 shows endogenous Cybr detected with antibody. Lane 3 shows no band detected in lysates prepared from splenocytes of Cybr knock-out mouse. Lane 4 shows direct western blot of wt splenocytes. Lane 5 shows direct western blot of knock out mouse.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Cytip.
Immunogen
A synthetic peptide corresponding to C-terminus of mouse Cytip.
Host
Rabbit
Theoretical MW (kDa)
40
Reactivity
Mouse, Rat
Specificity
Expect a band approximately 40 kDa in size corresponding to Cybr by western blotting in the appropriate cell lysate or extract.
Form
Liquid
Recommend Usage
ELISA (1:5000-1:20000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.02 M KH2PO4, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis using Cytip polyclonal antibody (Cat # PAB19941) shows detection of endogenous Cybr from mouse splenocytes using anti-Cybr antibody to immunoprecipitate and western blot (lanes 1-3). Lane 1 shows reactivity of pre-immune sera. Lane 2 shows endogenous Cybr detected with antibody. Lane 3 shows no band detected in lysates prepared from splenocytes of Cybr knock-out mouse. Lane 4 shows direct western blot of wt splenocytes. Lane 5 shows direct western blot of knock out mouse.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Cytip
Entrez GeneID
227929Gene Name
Cytip
Gene Alias
A130053M09Rik, AI462064, C80816, Cbp, Pscdbp
Gene Description
cytohesin 1 interacting protein
Gene Ontology
HyperlinkGene Summary
Sec7 and coiled-coil domains
Other Designations
OTTMUSP00000013590|cytohesin binding protein|pleckstrin homology, Sec7 and coiled-coil domains, binding protein|tamalin
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