Western blot analysis using Cytip polyclonal antibody (Cat # PAB19941) shows detection of endogenous Cybr from mouse splenocytes using anti-Cybr antibody to immunoprecipitate and western blot (lanes 1-3). Lane 1 shows reactivity of pre-immune sera. Lane 2 shows endogenous Cybr detected with antibody. Lane 3 shows no band detected in lysates prepared from splenocytes of Cybr knock-out mouse. Lane 4 shows direct western blot of wt splenocytes. Lane 5 shows direct western blot of knock out mouse.