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PDCD4 (phospho S457) polyclonal antibody

  • Catalog # : PAB19940
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of PDCD4.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S457 of human PDCD4.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 52
  • Reactivity:
  • Human, Mouse, Rat, Xenopus
  • Specificity:
  • This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
  • Form:
  • Liquid
  • Purification:
  • Immunoaffinity purification
  • Recommend Usage:
  • ELISA (1:400000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (21.25-2.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of recombinant protein with PDCD4 (phospho S457) polyclonal antibody (Cat # PAB19940). Lane 1& 2 each contain 100 ng recombinant PDCD4. Lanes 3& 4 each contain 30 ug of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteosome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of formalin-fixed paraffin-embedded human breast epithelial cell with PDCD4 (phospho S457) polyclonal antibody (Cat # PAB19940).
  • ELISA
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • PDCD4
  • Gene Alias:
  • H731,MGC33046,MGC33047
  • Gene Description:
  • programmed cell death 4 (neoplastic transformation inhibitor)
  • Gene Summary:
  • This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000020483,nuclear antigen H731,programmed cell death 4
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