Rabbit polyclonal antibody raised against partial recombinant NEDD1.
Recombinant GST fusion protein corresponding to amino acids 343-667 of human NEDD1.
Theoretical MW (kDa):
This antibody reacts with endogenous Nedd1 protein. A BLAST analysis was used to suggest reactivity with Nedd1 from human, chimpanzee, macaque, marmoset, cattle, rat, and mouse based on a 100% homology with the immunizing sequence. Expect partial reactivity with Nedd1 from turkey, chicken, salmon, and Danio based on a 91% homology with the immunizing sequence. Cross-reactivity with Nedd1 from other sources has not been determined.
ELISA (1:100000) Western Blot (1:10000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of S phase HeLa cells with NEDD1 polyclonal antibody (Cat # PAB19928) at 1:10000 dilution. Lane 1: lysates from sh-Luciferase lentivirus-infected cells as control; Lane 2: lysates from sh-NEDD1 RNAi-treated lentivirus-infected cells.