NOTCH3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NOTCH3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human NOTCH3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NIH/3T3 cell lysate (15 ug/lane) with NOTCH3 polyclonal antibody (Cat # PAB1984).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with NOTCH3 polyclonal antibody (Cat # PAB1984) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — NOTCH3
Entrez GeneID
4854Protein Accession#
NP_000426Gene Name
NOTCH3
Gene Alias
CADASIL, CASIL
Gene Description
Notch homolog 3 (Drosophila)
Gene Ontology
HyperlinkGene Summary
This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq
Other Designations
Notch homolog 3
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Interactome
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Pathway
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Disease
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Publication Reference
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Investigation of Notch3 as a candidate gene for bipolar disorder using brain hyperintensities as an endophenotype.
Ahearn EP, Speer MC, Chen YT, Steffens DC, Cassidy F, Van Meter S, Provenzale JM, Weisler RH, Krishnan KR.
American Journal of Medical Genetics 2002 Aug; 114(6):652.
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Combined expression of pTalpha and Notch3 in T cell leukemia identifies the requirement of preTCR for leukemogenesis.
Bellavia D, Campese AF, Checquolo S, Balestri A, Biondi A, Cazzaniga G, Lendahl U, Fehling HJ, Hayday AC, Frati L, von Boehmer H, Gulino A, Screpanti I.
PNAS 2002 Mar; 99(6):3788.
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Investigation of Notch3 as a candidate gene for bipolar disorder using brain hyperintensities as an endophenotype.
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