ZYX polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate with ZYX polyclonal antibody (Cat # PAB19818) at 1:1000 dilution.
Immunofluorescence
Immunofluorescence staining of HeLa with ZYX polyclonal antibody (Cat # PAB19818) at 1:50 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ZYX.
Immunogen
A synthetic peptide corresponding to 15 amino acids near internal region of human ZYX.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:32000)
Western Blot (1:500-1000)
Immunofluorescence (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate with ZYX polyclonal antibody (Cat # PAB19818) at 1:1000 dilution.Immunofluorescence
Immunofluorescence staining of HeLa with ZYX polyclonal antibody (Cat # PAB19818) at 1:50 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ZYX
Entrez GeneID
7791Protein Accession#
Q15942Gene Name
ZYX
Gene Alias
ESP-2, HED-2
Gene Description
zyxin
Omim ID
602002Gene Ontology
HyperlinkGene Summary
Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq
Other Designations
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Interactome
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Disease
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