PRKAA1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of MCF-7 cell lysate with PRKAA1 polyclonal antibody (Cat # PAB19817) at 1:200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed paraffin-embedded human breast carcinoma showing staining with PRKAA1 polyclonal antibody (Cat # PAB19817) at 1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKAA1.
Immunogen
A synthetic peptide corresponding to 15 amino acids near internal region of human PRKAA1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Peptide affinity purification
Recommend Usage
ELISA (1:40000-1:80000)
Western Blot (1:200-500)
Immunohistochemistry (1:50-200)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MCF-7 cell lysate with PRKAA1 polyclonal antibody (Cat # PAB19817) at 1:200 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed paraffin-embedded human breast carcinoma showing staining with PRKAA1 polyclonal antibody (Cat # PAB19817) at 1:100 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKAA1
Entrez GeneID
5562Protein Accession#
Q13131Gene Name
PRKAA1
Gene Alias
AMPK, AMPKa1, MGC33776, MGC57364
Gene Description
protein kinase, AMP-activated, alpha 1 catalytic subunit
Omim ID
602739Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
5'-AMP-activated protein kinase, catalytic alpha-1 chain|AMP -activate kinase alpha 1 subunit|AMP-activated protein kinase, catalytic, alpha-1|AMPK alpha 1
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Interactome
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Pathway
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Disease
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