AKR1C3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of human fetal liver tissue lysate with AKR1C3 polyclonal antibody (Cat # PAB19807) at 1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed paraffin-embedded human lung showing staining with AKR1C3 polyclonal antibody (Cat # PAB19807) at 1:100 dilution.
Immunofluorescence
Immunofluorescence staining of HeLa with AKR1C3 polyclonal antibody (Cat # PAB19807) at 1:50 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AKR1C3.
Immunogen
A synthetic peptide corresponding to 15 amino acids at C-terminus of human AKR1C3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Peptide affinity purification
Recommend Usage
ELISA (1:10000-1:40000)
Western Blot (1:500-1000)
Immunohistochemistry (1:100-500)
Immunofluorescence (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In serum (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of human fetal liver tissue lysate with AKR1C3 polyclonal antibody (Cat # PAB19807) at 1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed paraffin-embedded human lung showing staining with AKR1C3 polyclonal antibody (Cat # PAB19807) at 1:100 dilution.Immunofluorescence
Immunofluorescence staining of HeLa with AKR1C3 polyclonal antibody (Cat # PAB19807) at 1:50 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKR1C3
Entrez GeneID
8644Protein Accession#
P42330Gene Name
AKR1C3
Gene Alias
DD3, DDX, HA1753, HAKRB, HAKRe, HSD17B5, KIAA0119, hluPGFS
Gene Description
aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
Omim ID
603966Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq
Other Designations
OTTHUMP00000018996|aldo-keto reductase family 1, member C3|chlordecone reductase|dihydrodiol dehydrogenase 3|dihydrodiol dehydrogenase X|hydroxysteroid (17-beta) dehydrogenase 5|prostaglandin F synthase|trans-1,2-dihydrobenzene-1,2-diol dehydrogenase|type
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