NUP84 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NUP84.
Immunogen
A synthetic peptide correspoding to N-terminus ofyeast NUP84.
Sequence
ELSPTYQTERFTKC
Host
Goat
Theoretical MW (kDa)
83.6
Reactivity
Yeast
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
NUP84 polyclonal antibody (Cat # PAB19763) (2 ug/mL) staining of Saccharomyces cerevisiae S288c lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Data kindly provided by Dr. F Reggiori, University of Utrecht, Netherlands.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NUP84
Entrez GeneID
851441Protein Accession#
NP_010167.1Gene Name
NUP84
Gene Alias
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Gene Description
Nup84p
Gene Ontology
HyperlinkGene Summary
forms a subcomplex with Nup85p
Other Designations
Subunit of the nuclear pore complex (NPC), forms a subcomplex with Nup85p, Nup120p, Nup145p-C, Sec13p, and Seh1p that plays a role in nuclear mRNA export and NPC biogenesis
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