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NOX1 polyclonal antibody

  • Catalog # : PAB19751
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of NOX1.
  • Immunogen:
  • A synthetic peptide correspoding to internal region of human NOX1.
  • Sequence:
  • CDKATDIVTGLKQK
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 64.9
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody is expected to recognize both reported isoforms (NP_008983.2; NP_39249.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:16000)
    Western Blot (1-3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • NOX1 polyclonal antibody (Cat # PAB19751) (1 ug/mL) staining of MASM cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. NOX1 served as a loading control.
    Data provided by Dr. B. Lasseque, Emory University, Atlanta, USA.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_008983.2;NP_39249.1
  • Gene Name:
  • NOX1
  • Gene Alias:
  • GP91-2,MOX1,NOH-1,NOH1
  • Gene Description:
  • NADPH oxidase 1
  • Gene Summary:
  • Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • NADPH oxidase 1 variant NOH-1L,NADPH oxidase homolog-1,OTTHUMP00000023657,OTTHUMP00000023659,mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating)
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