NOX1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
NOX1 polyclonal antibody (Cat # PAB19751) (1 ug/mL) staining of MASM cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. NOX1 served as a loading control.
Data provided by Dr. B. Lasseque, Emory University, Atlanta, USA.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NOX1.
Immunogen
A synthetic peptide correspoding to internal region of human NOX1.
Sequence
CDKATDIVTGLKQK
Host
Goat
Theoretical MW (kDa)
64.9
Reactivity
Human, Mouse
Specificity
This antibody is expected to recognize both reported isoforms (NP_008983.2; NP_39249.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
NOX1 polyclonal antibody (Cat # PAB19751) (1 ug/mL) staining of MASM cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. NOX1 served as a loading control.
Data provided by Dr. B. Lasseque, Emory University, Atlanta, USA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NOX1
Entrez GeneID
27035Protein Accession#
NP_008983.2;NP_39249.1Gene Name
NOX1
Gene Alias
GP91-2, MOX1, NOH-1, NOH1
Gene Description
NADPH oxidase 1
Omim ID
300225Gene Ontology
HyperlinkGene Summary
Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
NADPH oxidase 1 variant NOH-1L|NADPH oxidase homolog-1|OTTHUMP00000023657|OTTHUMP00000023659|mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating)
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Interactome
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Pathway
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Disease
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