UBE2F polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of UBE2F.
Immunogen
A synthetic peptide corresponding to N-terminus of human UBE2F.
Sequence
SKLKRDDGLKGSRT-C
Host
Goat
Theoretical MW (kDa)
21.1, 18.6
Reactivity
Rat
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:32000)
Western Blot (0.05-0.2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
UBE2F polyclonal antibody (Cat # PAB19723) (0.05 ug/mL) staining of rat pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — UBE2F
Entrez GeneID
140739Protein Accession#
NP_542409.1Gene Name
UBE2F
Gene Alias
MGC18120, NCE2
Gene Description
ubiquitin-conjugating enzyme E2F (putative)
Gene Ontology
HyperlinkOther Designations
NEDD8 conjugating enzyme|NEDD8-conjugating enzyme
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Interactome
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Pathway
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